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The intervention did not have a statistically significant effect on the total physical activity, moderate-to-vigorous physical activity, or sedentary time. The intervention resulted in a significant increase in light physical activity among the participants in the experimental group at the six-month follow-up (+1.07 min/h, an 11.16% increase p = 0.0017). Results: A total of 148 children (31.92 ± 7.41 months) had valid accelerometry data. An adjusted alpha ( p < 0.017) was used to account for multiple comparison bias. The participants with two or more days of at least 5 h/day of wear-time at baseline, and at one additional time point, were included in the linear mixed-effects models. Raw accelerometry data were converted to 15 s epochs, and age- and device-specific cut-points were applied. To assess physical activity levels, toddlers and preschoolers wore ActiGraph wGT3X-BT accelerometers for five consecutive days during childcare hours, at baseline, mid- and post-intervention, and at the six-month follow-up. The centers in the control condition ( n = 4) continued their typical daily routines, while the centers in the intervention condition ( n = 5) implemented the PLAY policy for eight weeks. Methods: Nine childcare centers in London, Ontario participated in the cluster, randomized controlled trial. The purpose of this study was to examine the impact of the Childcare Physical Activity (PLAY) policy on the physical activity and sedentary time of young children (18 months-4 years) in childcare. With eight recommendations, the Childcare PLAY policy is an evidence-informed, institutional-level document, targeting children's physical activity, outdoor play, and sedentary time. Currently, a formal written physical activity policy is lacking in Canadian childcare centers, but holds promise for offering consistent physical activity opportunities. All Rights Reserved.Background: The importance of daily physical activity is crucial for healthy development during the early years.
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